Volume 36, Number 1, February 2018
|Page(s)||132 - 138|
|Published online||18 May 2018|
A Novel Single Cell Culture Chip Based on One-Step Method
School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an 710072, China
2 Key Laboratory of Micro/Nano Systems for Aerospace, Ministry of Education, Northwestern Polytechnical University, Xi'an 710072, China
3 Institute of Electronic Engineering, China Academy of Engineering Physics, Mianyang 621000, China
4 School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China
5 Key Laboratory of Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Northwestern Polytechnical University, Xi'an 710072, China
More and more basic biology research focus on the single cell level. However, the way to fabricate a chemically patterned surface exist some questions such as complex fabrication process, graphic quality puzzle and so on. In this paper, we present a One-Step method based on Poly 2-hydroxyethyl methacrylate (PolyHEMA) and micromolding in capillaries (MIMIC) technology to fabricate single cell culture chip. Experimental evidence indicates that the integrity and stability of the PolyHEMA pattern can perfectly satisfy the need of long-term cell culture.
越来越多的生物学研究聚焦在单细胞层面，然而现有基于化学表面修饰的单细胞定位培养芯片存在制备工艺复杂，且细胞定位层图形质量较差影响细胞定位效果的问题。为此，提出一种一步成形的方法，利用聚甲基丙烯酸2-羟乙酯（poly 2-hydroxyethyl methacrylate，PolyHEMA）抑制细胞粘附的特性并结合毛细微模塑技术制备芯片。细胞验证结果表明，该芯片具有制备过程简单，图形完整性和稳定性良好的特点，为基于单细胞的生物学研究提供了一种新方法。
Key words: single cell culture / micropattern / MIMIC / PolyHEMA
关键字 : 单细胞培养 / 图形化 / 毛细微模塑技术 / PolyHEMA
© 2018 Journal of Northwestern Polytechnical University. All rights reserved.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.